FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10340307
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG20200.017.0964721
GTACTAG12900.015.2595951
CGCTAGA3654.3655746E-1115.1329362
GTCCTAA24100.015.0463141
GTCCTAG26000.015.0093881
GTCCTAT22800.014.6924681
GTATTAA30650.014.4223941
GTTCTAG29200.013.95587351
GTGCTAA24900.013.8694281
GTACTAC15650.013.6815481
CTAGACT21700.013.6766374
GTCTTAG22800.013.6321871
GCGCTAG3557.237759E-913.6193881
GTACTAT20550.013.6122871
CTAAGCG7200.013.4204224
GTACTAA18600.013.3683391
GCGTTAG3701.2929377E-813.0672481
GTATAGG17350.012.9381371
GTATAAG19600.012.6862811
GCTCTAG28050.012.6812561
Produced by FastQC (version 0.11.5)