FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10297998
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19650.016.9269941
GTACTAG12350.015.3089571
GTACTAA18400.015.2226751
GTACTAT19350.014.4753071
GTTCTAA27100.014.3407711
GTATTAA29800.013.8638731
GGTCTAA19700.013.86267951
GTCCTAA22250.013.5327861
GTCCTAG26150.013.5228571
ATCTCCG117800.013.36963170
GTATAGG18100.013.1537361
CGATCGT4355.7298166E-1012.87684438
GTGCTAG26650.012.7436341
GTCTTAA28800.012.5217071
GTGTTAG26500.012.4194041
GGTCTAG21750.012.0731551
GTCCTAT20700.012.0089981
GCGCTAG4104.4719854E-811.9553681
TATACTG30300.011.8982365
GTTCTAG29700.011.7886041
Produced by FastQC (version 0.11.5)