FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10297998
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG26250.017.604921
GTACTAG11950.017.2851641
GTATTAG21150.016.7186181
GTGCTAG26150.015.9317931
GTACTAA19600.015.3614351
GTTCTAG28300.014.4740091
GTTCTAA27700.014.0291891
GGTCTAG23550.013.5281951
GTCCTAA21100.013.2738511
GGTCTAA18750.012.8835991
GTATAAG20150.012.8571911
GTATTAA29850.012.7841411
GCCCTAG29650.012.6342211
GTCCTAT21700.012.5841611
TCTGACG93950.012.47667670
GTACTAT19400.012.4519331
GTATAGA22000.012.2534231
GATCTAG17800.012.1944181
GTATATA36100.012.12251
CCTATAC14950.011.9331023
Produced by FastQC (version 0.11.5)