FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10096886
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11600.017.2037141
GTCCTAG24200.016.2034681
GTACTAA17150.016.1275461
GTATTAG20250.015.0417921
GTTCTAG29700.013.5564741
GTCTTAG22900.013.4540341
GTGCTAA24750.013.4385911
GGTCTAA17500.013.4042381
GTCCTAA22550.013.0418171
GTATATA38150.012.939871
CGATCGT3801.557055E-812.89472738
ATCTCCG115200.012.63862770
GTACAGG43900.012.5210431
GCTCTAG29350.012.4059661
GTATTGG24100.012.3483031
GTACTAC14750.012.3428851
GTATTAA28100.012.3348591
GTATATG29300.012.3076441
TCTATAC20700.012.0057723
TCTCCGA96650.011.913877570
Produced by FastQC (version 0.11.5)