FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10096886
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG27600.016.7399561
GTACTAA19250.016.7280981
GTCTTAG22950.015.7088361
GTGCTAG26250.015.600771
GTATTAG20550.014.9885741
GTTCTAA28900.013.8069091
GTGTAAG24850.013.8034971
GTCCTAT20800.013.7987581
GTTCTAG28950.013.7830641
GTCCTAA22400.013.7506781
GTACTAG13050.013.6788351
TTGCGCG3951.879016E-913.29074212
GGTCTAA19950.013.1585441
TCTAGAC21200.013.0394133
GTATAAG20450.013.0079771
GTGCTAA22250.012.8995131
TAGTACG1900.001896772812.8923714
GTATATG27300.012.6929341
CTAAGCG5800.012.670094
GTATTAA26600.012.6322021
Produced by FastQC (version 0.11.5)