FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9812146
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG10900.016.7052961
GTATTAG19950.014.7439461
GTCCTAG26150.014.5959421
GTATATA35600.013.3772321
GTATAGG15500.013.1030861
GCTCTAG27300.013.0832271
ATCTCCG112100.013.08150670
GTTCTAG27650.013.0442591
GTACATA23900.013.0397561
TCAGTAC26000.012.9245623
GTCCTAA20150.012.8597951
TATACTG27600.012.8094435
GTCTTAG23350.012.7470411
GTGTTAG28450.012.6774611
GGTCTAA18000.012.6449811
GTGCTAG23950.012.5739081
GTCTAGA24250.012.5627531
GGTCTAG20350.012.5613351
TCTCCGA91400.012.368175570
GTATATG28650.012.3445171
Produced by FastQC (version 0.11.5)