FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9812146
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11000.020.0415741
GTATTAG19650.016.2055151
GTACTAA18850.015.7794391
GTTCTAG27600.013.9465751
GGTCTAA18750.013.8106591
GTATACG3556.013579E-913.8001441
GTACATA27350.013.690221
GGTCTAG21000.013.4973871
CGATCGT3701.0702934E-813.24466338
GTGCTAG26300.013.1723561
GTATTAA27900.013.1694941
GCGCTAA3751.2971213E-813.0641371
TCTGACG87550.012.95299670
GTCCTAA21350.012.9483141
GTACTAT16900.012.6306911
GTCCTAG24650.012.4925111
GTATATA36250.012.4527621
CAATACG5701.8189894E-1212.2776374
GTCTATA19800.012.1946091
TCTAGAC22450.012.0003573
Produced by FastQC (version 0.11.5)