FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11364061
Sequences flagged as poor quality0
Sequence length79
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19900.017.428891
GTCCTAG33450.016.4808651
GCGAGAC48250.016.4163863
GTATTAG33700.015.816931
CGAGACT48550.015.6382884
GGTCTAG23400.015.2900691
GTGCTAG37200.014.9176311
GAGCGAG53500.013.7846841
GTACAGG44350.013.5828081
GTACTAA29800.013.4764531
AGCGAGA56400.013.267292
GGTCTAA24300.013.2213441
GTTCTAG40000.013.1432171
GCGCTAG5001.4551915E-1113.1432171
GTGTTAG44050.013.0951471
GTACAAG37150.013.0704951
GTCCAGA56150.013.0040741
CTATACT39900.012.6247074
GTGCAGA76600.012.5827151
GTGCAGG81950.012.5631731
Produced by FastQC (version 0.11.5)