FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11089000
Sequences flagged as poor quality0
Sequence length79
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18250.018.3994311
GTCCTAG32750.016.6055951
GTATTAG35850.016.5950241
GTGCTAG34550.015.6348291
GTTCTAG39100.015.2156421
GCGAGAC45650.014.3906313
GGTCTAG24050.014.2656721
GTCTTAG34300.014.2590351
CGAGACT48400.014.2515674
GTTCTAA40550.013.77146151
GTCCTAT25500.013.7407511
GTGTTAG42750.013.575021
GTACTAA29900.013.5497481
GTCCTAA29400.013.1594571
GGTCTAA24200.013.1214961
GTATAGG20650.012.9027491
GTGCAGA74600.012.8186391
GTCTTGC56800.012.5946821
GTCTCGC17400.012.5858181
GAGCGAG51350.012.5809171
Produced by FastQC (version 0.11.5)