FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16992711
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG29500.017.327651
GTACTAG20650.017.2937411
GTATTAA47150.016.3361551
GTCCTAG39000.015.7999941
GTCTTAG37000.014.4776661
GTATAGG26850.014.2131741
GTATATA56150.014.1541871
GTACTAT30900.014.0498621
TATACTG51450.014.0185635
GGTCTAG30850.013.9591451
GTTCTAG44450.013.9415271
CTATACT38000.013.6362814
GTATAAG31500.013.3376571
GTACTAA30300.013.28813651
GTTCTAA45650.013.2682671
TACGCGA4002.2555469E-913.1294112
GTGCTAG37950.013.1004261
GTCTTAA45050.013.0563971
TCTATAC36050.013.0140813
TGCACTG113500.012.7710365
Produced by FastQC (version 0.11.5)