FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16889060
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG31250.019.2652931
GTCCTAG40600.017.4149631
GTACTAA31000.015.6946021
GTACTAG19050.015.4341081
GTATTAA46400.015.3135291
GTTCTAG46300.014.81740951
GTCTTAG35000.014.000941
GTATATA56200.013.9511151
GTATAAG32550.013.7643651
GTACTAT31650.013.7134021
GGTCTAA32450.013.6989161
GTGCTAG40450.013.6721171
GCTCTAG50700.013.4624421
CTATACT39200.013.215334
TCTATAC36900.013.1853233
GTTCTAA44800.012.6571011
CGATCGT6650.012.63160538
GTACTGG69300.012.5766031
GTCCTAA36550.012.5453021
GTCTTAA43500.012.4721021
Produced by FastQC (version 0.11.5)