FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16889060
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG40850.017.3394371
GTATTAG32000.016.984681
GTATTAA47750.016.302541
GTACTAG20500.016.2497021
GTTCTAG45900.016.1192741
GTGCTAG41300.016.0467621
GGTCTAG32850.015.4777681
GTCTTAG36450.014.8149051
GTATATA55050.014.5228951
GTACTAA30800.014.3448331
GCTCTAG47200.013.8180471
GTATTGG41900.013.8084721
CTATACT37250.013.7178994
GTGCTAA39250.013.4900311
GTTCTAA46800.013.4865951
GTCTAGG40400.013.3664191
GTCCTAT33850.013.3630851
GTCCTAA34400.013.3532981
CCTATAC26850.013.299973
GTACAGG77150.013.271581
Produced by FastQC (version 0.11.5)