FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16712833
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG29950.016.9473081
GTCCTAG40700.016.5994011
GTTCTAG45100.015.4456451
GTACTAG17700.015.4259131
GTATTAA50450.014.7097131
GGTCTAA31750.014.6634931
GTACTAA30550.014.4373951
GTATATA56350.013.8528931
GTGCTAG39200.013.573351
GTTCTAA44000.013.4450861
GTCTTAG39250.013.2885061
GTCCTAA34700.013.2151281
TACGCGA4801.4551915E-1113.1266622
GTGTAGA63750.013.0685161
GTATAAG31150.013.0355541
TCTATAC35600.012.9793263
CTATACT36150.012.9756744
TATACTG48700.012.9384475
GTACAGG76250.012.8083711
GTATAGG26800.012.8003191
Produced by FastQC (version 0.11.5)