FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16712833
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG42050.017.6566261
GTGCTAG39300.017.1098631
GCGATCG7200.016.528337
GTATTAG31350.016.3099691
CGATCGT7100.016.26811838
GTACTAG19350.015.9272041
GTTCTAG46050.015.7426751
GGTCTAG31450.015.5899681
GTCTTAG33750.015.5652231
GTATTAA46450.014.9285391
GTTCTAA47950.014.8267251
GTCCTAA34900.014.1491871
GTATAGG25250.014.00871
GGTCTAA33500.014.0086991
GCTCTAG50750.013.8706831
GTATATA53600.013.2638331
TCTAGAC37000.013.2421543
GCCCTAG39950.013.2372561
GTGCATA39700.013.2323981
TCTGTAC76800.013.0783523
Produced by FastQC (version 0.11.5)