FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16634200
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19750.018.2626481
GTATTAG30800.016.599531
GTGCTAG39500.016.400921
GTTCTAG45000.015.7970921
GTCCTAG40150.015.6993091
GTTCTAA49300.015.5557451
GTACTAA31400.015.0555891
GTCCTAA34850.014.9719071
GTCTTAG36000.014.7854571
GTATAGG27000.014.526061
GGTCTAA32600.014.5013951
GTATTAA47000.014.3798071
GTGCTAA39150.013.6852671
GCTCTAG50300.013.5756391
GTACAGG75350.013.3380481
GTATTAT48950.013.2346561
GTATATG47550.013.1824491
GTCTTAA44550.013.1269061
GTATATA53600.013.0664851
TGTACTG67600.012.8402275
Produced by FastQC (version 0.11.5)