FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10557724
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG76350.025.923685145
GTCCTAG42950.022.2855741
TGCCGTG87350.021.082102145
GTACTAG19050.020.5547331
GCGAATG155250.020.17415145
GTATTAG33300.018.9447141
GTATAAG34850.017.8940531
GTATAGG29000.017.0029371
GTATATA57500.016.8985711
TCTATAC37150.016.7835883
GTACTAA33250.016.356211
GGGGGGG4796850.016.22365145
GCCCTAG43650.015.9477721
GTATAGA39350.015.8477181
GTTCTAG46750.015.8209151
GTCTTAG43100.015.8147971
GTGCTAA39500.015.7875391
GTGTAGG52650.015.7007191
GTGTAGA57550.015.62389851
GTTCTAA47350.015.6204391
Produced by FastQC (version 0.11.5)