FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10557724
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT19750.034.138138145
CCGTATA21650.027.458694145
GTACTAG19300.021.7954311
GTCCTAG41350.021.5736851
TTATACC110850.019.685877145
GGTCTAA32200.019.3703421
GTCCTAA36500.019.2740781
GTCTTAG40250.018.7396811
GTATTAG34900.018.7030241
GTATAGG29350.018.0388761
GTTCTAG50000.017.986491
TCTATAC41400.017.6827723
GTATTAA51300.017.672071
GTGCTAG43850.017.3665851
GCTCTAG51050.017.3324071
GTACTAT33050.016.8971711
GTTCTAA49550.016.5397741
GTACTAC28550.016.5120871
GTACTAA30600.016.3539411
CTATACT38100.016.1708764
Produced by FastQC (version 0.11.5)