FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10607147
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT20700.027.315866145
CCGTATA21200.026.671623145
GTCCTAG42150.020.6471821
GTTCTAG49300.020.4477251
GTATAGG28500.019.5939941
GTACTAT33800.019.3109191
GTCTTAG40650.018.5545271
GTATTAG34350.018.1571961
GTACAGG80100.017.7460081
GCTCTAG46000.017.6578271
CTATACT38500.017.5130634
TTATACC107800.017.416935145
GTGCTAG43800.017.0545481
GTCTATA34600.016.5587711
GGTCTAA34200.016.328331
GTACTAA31150.016.0645351
GTACATA47150.015.9966391
CTAGACT36250.015.8000684
GTCCTAA38250.015.7370651
GGTCTAG32550.015.5963931
Produced by FastQC (version 0.11.5)