FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155018
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG1409.872087E-619.59052837
CGATCGT1501.7873266E-518.28703938
GTACTAT3350.017.398771
GTCGTAC1200.001540256817.1429061
GGATTAG4600.016.397561
GTATTAA4900.016.093341
GTATAAG3900.015.824221
GTACGGT1300.00262968615.824221
GGTTTAG4600.015.6522171
GTTCTAG5700.015.6391421
CTAATAG3101.1823431E-915.48463253
GTCCTAG5150.015.3121111
GTGCTAG4750.015.1579371
GGTCTAG4350.014.975411
GTACTAA3909.094947E-1214.9450971
GGTCTAA4650.014.7465851
ATTAGAC3757.4578566E-1114.62929253
CTATACC3809.276846E-1114.4371374
TCTATAC3809.276846E-1114.4368013
GTACATA4750.014.4361311
Produced by FastQC (version 0.11.5)