FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2130398
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG450.003617023832.2441663
CTTAACG1403.9593033E-722.0714133
GTACTAG2401.8189894E-1221.4911521
GTATTAA5850.019.3971421
CGTTAAC900.005898743419.074063
TCTATAC3400.017.1666553
GTACTAA4250.016.1815741
GTATTAG3101.1350494E-915.5290911
GTCCTAG5400.015.2825981
GTATAAG4750.015.2021621
GTCTTAG4300.015.1937451
TAGACTG5900.015.109695
ATTACAC4201.8189894E-1214.7142763
TACACTA2606.1407627E-714.5061975
TAGACTA1901.3460238E-414.4439094
TTGTTCG2450.00489040914.41485770
GTCTATG5750.014.3523511
CTAGGCC5500.014.3454284
GTCTTAA4102.0008883E-1114.2575451
GTTGCGC1709.5634005E-414.11700511
Produced by FastQC (version 0.11.5)