FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180304
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG2000.027.4257761
GTCTATA3950.019.9617981
GTATTAG3800.019.84761
CGAATCG1259.202389E-519.192329
GTCCTAG4450.018.4892861
GTATATG7300.017.845541
TAGTACA4350.017.3381354
CGTATAC1402.1869423E-417.141112
CTTAGCG1250.002025293716.4554673
GTACTAA3601.8189894E-1216.1888261
GTATAGG3201.07320375E-1016.069791
GGATATA4750.015.878081
CTGTACT10200.015.7967094
CTAGGCC5050.015.6136854
TCTAGGC6400.015.534133
CTAACCT5350.015.3789394
ATTATAC4700.015.3175873
GTTCTAG5150.015.3105061
TGTACTA4750.015.156355
GGTCTAG4001.2732926E-1114.5699431
Produced by FastQC (version 0.11.5)