FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2134810
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG5150.020.6419011
GTTCTAG6000.018.2891671
GTACTAA2851.8189894E-1118.048521
GGTCTAG3750.017.3747081
GTCTTAG3400.017.1460951
TACTAGG3055.2750693E-1116.8650112
CTATACT4650.016.2242624
CGAATCG1503.619153E-416.04549645
GTGTATA4300.015.9498561
GTATTAG2908.163624E-915.3723611
GTACTAG2252.182669E-615.2409731
ATAAGCG1901.0991456E-414.79191256
GTCCTAA4650.014.749331
CTAAGCG1657.615873E-414.5482014
GTACGGA1657.615873E-414.5482011
CCCTATA2606.1310493E-714.5082351
TTATACT5250.014.3700614
CTAGTAC2404.2655356E-614.2884123
GTTATAG4102.0008883E-1114.2187131
CGCGAGA1450.00543605414.18754617
Produced by FastQC (version 0.11.5)