FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2134810
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA800.0057146529.36522970
GCGTTAG850.00425296720.1841931
CGATCGT1108.574569E-418.70704338
TTAGTAC2255.973561E-918.288763
GTCTTAA4200.017.9735411
TCTATAC4850.017.6759933
CGAATCG1402.139066E-417.19081745
GTACTAA3055.0931703E-1116.8753071
GCTCTAG6150.016.738111
GTATAGG3551.8189894E-1216.4316391
GTATTAG3653.6379788E-1215.9814561
CTATACT4650.015.4857244
GTATATA4700.015.3313971
GTCCTAG5000.015.0977761
GTGTACG2503.906316E-715.0977761
GCCTTAA4650.014.7583351
GTATTAA5000.014.4115141
GTACTAG2152.3837269E-514.3636341
CCTAGTA2951.6697777E-713.9513412
GTCTTAG5200.013.8572241
Produced by FastQC (version 0.11.5)