FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4524391
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA8550.018.0150661
GTATTAG4950.017.2871841
GTACTAA6800.017.1143131
TCTATAC7650.015.6605013
GGTCTAG7850.015.2611691
CGATCGT2705.7603756E-815.219954538
TACTAAC6250.014.7869282
TTTAGAC9750.014.7449643
GTTCTAG11200.014.669411
GGTTTAG8800.014.3915811
GTGCTAG10150.014.16356851
CTATACT8600.013.9307164
ACTAACC8700.013.7704413
GTATATG10950.013.7539671
TAATACT10750.013.6919034
GTCCTAG10200.013.4229911
GTATAAT7400.013.413921
GTGTATA8950.013.3854951
CCTATAC6950.013.2977463
GCGATCG3103.1192394E-713.25476937
Produced by FastQC (version 0.11.5)