FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4524391
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG4800.020.7311041
GTATTAG5900.019.7739121
TAGTACG1552.3988066E-517.6728254
CTATACT8700.017.3173354
GTTCTAG9950.016.208411
GTGCTAG10650.016.109651
GGTCTAA7300.015.9816551
TAGTGCG2553.5284393E-715.23486257
GTATTAA8800.015.2071421
CTAGACT7450.015.1671974
TATACAG10950.015.0061455
CCCCTAG11000.014.97318651
GTACTAA6900.014.9189361
CGTTAAG1657.582343E-414.5572641
GTGCTAA9450.014.5242551
GTCCTAG10350.014.2558711
CCTATAC7150.013.8964463
GTATAAG6700.013.8278491
GTATATA8950.013.8020981
GTCTTAT9700.013.796171
Produced by FastQC (version 0.11.5)