FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4397432
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAA8450.018.2742881
TTGCGCG2058.4311796E-716.69755712
GGCGTAT1907.3962583E-616.2544981
GTCCTAG10000.016.1280751
CTAGTAC4200.015.501743
GTCCTAT8450.015.4316211
TCTAGAC7200.015.2297813
CGAATCG2404.349049E-614.2624979
GTGCTAG9700.014.1505371
GTTCTAG9600.013.940491
GTATTAG6900.013.9249471
GTATATA9200.013.8006171
GCGCTAA1500.006769381513.7260191
GCTCTAA11750.013.4339771
GTATAAG6200.013.2832451
GCGCTAG1550.00840873413.2832451
GTGTTAG8650.013.091291
GGTCTAG7350.013.0723991
GTACTAA7900.013.0310331
GTACGGG6400.012.8681431
Produced by FastQC (version 0.11.5)