FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8072513
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA14850.016.95969873
GTCCTAG19300.015.7030191
CCGACGA21050.014.73880773
GTATTAG15750.013.9101891
GTTCTAG23200.013.8502311
TGACGCT113550.013.597177573
GTATAGG12500.013.4372431
GTCCTAA16650.013.3775921
GACGCTG103800.013.256824573
GTATTAA23350.013.1357461
GTGCTAG20600.012.9395151
GTACTAA15550.012.9150071
TTGCGCG2851.3222163E-512.8066212
CTGACGC139100.012.80525573
GCCGACG28500.012.55095473
GTTCTAA24500.012.519171
GTACAGG34250.012.2602581
GTCTTAG18850.012.20371
ACGCTGC76950.012.19045773
GTATTAC16550.012.1346431
Produced by FastQC (version 0.11.5)