FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8177801
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA15600.014.5073561
GTCTTAG20050.014.0183711
GTTCTAG23600.013.9203991
GTCCTAG20200.013.9142731
TCCGAGC111350.013.70181273
CGAGCCC73600.013.53869373
GTCTAGA18700.013.4687921
GTATTAG15750.013.4421451
GGTCTAA14850.013.2735931
CTAGACT17550.013.1035794
CCGAGCC93000.012.87309273
GTACGTA4556.020855E-1012.8360011
TCTGTAC35400.012.6831073
GTCCTAA19050.012.0716521
CTGTACT39550.011.9984024
GTATATG24200.011.9160661
GTATTAA26950.011.7836991
GTGCTAG20150.011.77496051
GTGTAGA29650.011.6955341
GTACTAC14400.011.6604821
Produced by FastQC (version 0.11.5)