FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8033648
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG101320.12611954120967211No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG20500.017.6458381
GTACTAG9850.017.0640681
GTGCTAG19200.015.7956621
GTCTTAG19200.014.6538071
GGTCTAG15200.014.1830411
GTGCTAA21050.013.8866941
CGACGAA15850.013.81850173
TGACGCT111200.013.75460673
GTATTAA24000.013.2455191
GTACTAA14900.012.9972221
ACGCTGC78050.012.9084873
CTAGACT17000.012.8862074
GACGAAT16900.012.74395673
GTTCTAG23800.012.5891911
TCTAGAC17200.012.5258883
GTATATA32200.012.3689151
GACGCTG101900.012.359014573
CTGACGC133600.012.15885173
GTGTTAG20500.012.1203731
GGTCTAA16100.012.0284861
Produced by FastQC (version 0.11.5)