FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6442629
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG7750.015.56279851
GTTCTAG18750.015.2545061
GTACTAA13400.015.1728981
GTATAGG9850.014.3439561
GTCGTAC3404.0654413E-914.18961051
GTATAGA14350.013.9282781
CTAGCGA1500.00658053913.7849414
GTCCTAA14750.013.5505621
GTCCTAG16300.013.5304951
GTATATA22650.013.3886191
TCTATAC13650.013.1285153
GTACATA17100.013.0990131
CTAGCGC2406.441446E-512.9233824
GTGTTAG17300.012.7483851
GTATAAG13500.012.5078781
GTATTAG13450.012.2981641
GTATACG2255.4185005E-412.2526151
GTACTAT12400.012.2279121
GTGCTAG13800.011.9862551
GTACAGA30400.011.9024691
Produced by FastQC (version 0.11.5)