FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6442629
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG12700.017.1376131
GTGCTAG15550.016.4404931
CGCGATT1400.002308500516.13682268
GTATAGG10800.015.3543351
GTCCTAG15650.015.2316961
GTATTAA20050.014.4736621
GTACTAA12500.014.3716571
GTACTAG7450.013.9116451
GCCCTAG15100.013.4985951
GTCTTAA18250.013.4403021
CTAGACT13850.013.4402924
GTACATA17050.012.9678841
GCTCTAG19450.012.9663211
GTGCATA14400.012.955221
GTCTTAG14500.012.8658741
GTTCTAG17150.012.6908271
GGTCTAA12850.012.6359611
CGATCGT2204.4871905E-412.53006438
CCCCTAG15200.012.5006511
TATACTG19450.012.399685
Produced by FastQC (version 0.11.5)