FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6355670
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG8100.018.7583261
GTATTAA19950.015.7516081
GTATTAG12950.015.7328951
GTCCTAA14750.015.6858921
GTCCTAG16050.015.4911581
GCGTTAA2252.0313346E-615.3477211
GTTCTAG19000.015.2669431
GTTCTAA19950.014.5399471
GTGCTAG14300.014.24762251
TCTAGAC13850.013.94113
CTAGACC12500.013.7813124
GTCTTAG14550.013.7654821
GTCTAAG13050.013.7600261
GCTCTAG17100.013.732171
CTAGACT11150.013.5959134
GGTCTAA12500.013.260431
GTATACG3153.4195364E-713.1551891
GTCTATA11600.013.0984861
GCCTTAG14350.012.994761
CTAGTAC6700.012.8654213
Produced by FastQC (version 0.11.5)