FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6463395
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG13050.016.3644661
CGATCGT2201.6498325E-615.65746838
GTACTAG7400.014.8949451
GTATTAA20550.014.7499841
GTACTAA13800.014.7262991
GTTCTAA18350.014.2658691
GTCCTAA13800.013.9775041
GTCTTAG14900.013.8702931
GTATTAT19550.013.7425891
GTATATA22950.013.6577571
GCTCTAG18000.013.3951091
GTCCTAG15350.013.2392771
TCTAGAC13400.013.10973
GTACATA17950.013.0486371
CTACTAG10350.012.9791121
GTATATG22200.012.8779231
TATACTG20400.012.8326855
CTATACT16300.012.6793734
GTTCTAG19300.012.6713171
GTATAGG11300.012.4975841
Produced by FastQC (version 0.11.5)