FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6263207
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG7800.018.1176011
GTCCTAG15300.017.3464571
GTGCTAG13800.015.984991
GGTCTAG12900.015.4970761
GTACTAA11850.013.9615731
GTCTTAA16900.013.8686341
GTATTAA20350.013.7193031
GTATTAT18600.013.342311
GTTCTAG18550.013.1924631
GTATATA20800.013.0910721
CTAGACT13900.012.885414
GTACATG23950.012.8084321
GTCTTAG13400.012.6038371
TCTGTAC26900.012.5491613
TATACTG19000.012.5078995
GTACTAC9550.012.2711981
GTCCTAA12650.012.2612131
GTATTAG13250.012.2262541
CTAAGCG3953.342211E-812.2078914
GTACAGA30300.012.1717381
Produced by FastQC (version 0.11.5)