FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13114695
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16850.017.6702841
GTCCTAG32450.017.284041
GTATTAG28500.015.4277951
GTCTTAG31900.015.0858251
GCCCTAG32950.014.7101671
GTTCTAG38450.014.6769651
TACGCGA2605.562579E-714.6351022
GTACTAA24700.014.156941
GGTCTAA24500.014.1311951
GGTCTAG27000.014.1050261
GTATTAA44550.013.9884561
GTCCTAA28400.013.8973331
GTTCTAA41000.013.7641281
CTAGTAC15550.013.560033
GTCTAGA29700.013.5221731
CTATACT31300.013.2438134
GTCCTAT30150.012.8610281
GTGTTAG34650.012.7894461
GTACAGA60950.012.7806751
CCTATAC22300.012.7107223
Produced by FastQC (version 0.11.5)