FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13191499
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG33550.016.6264341
GTACTAG15750.015.6186511
GTGCTAG31850.015.4470181
GTATTAG26750.015.1540181
GTTCTAG42050.014.99585151
GTATAGG20500.014.7038531
GTACTAA25000.014.69033051
GGTCTAA25250.014.2704511
GGTCTAG25300.014.1053041
GTCTTAG32700.013.9859481
GCGCTAG5950.013.39296151
GTCCTAA28700.013.2793411
GTATATA50950.013.0563791
GTACAGG59650.012.6041931
GGTCTAC21250.012.5544441
TCTATAC29250.012.531723
TCTAGGC38900.012.4454083
TCTGTAC51600.012.3980323
GCTCTAG38250.012.3189361
CTAGACT29500.012.1806264
Produced by FastQC (version 0.11.5)