FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11607983
Sequences flagged as poor quality0
Sequence length79
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG21650.020.067161
GTCCTAG35500.018.6143171
GTCTTAG32850.017.559761
GTATTAG38050.017.5587371
GTTCTAG42950.017.085581
GCGAGAC41350.015.8922853
CGAGACT42350.015.6894374
GTACTAA31250.015.6549481
GCCCTAG33200.014.6254891
GTATTAA55800.014.2632711
GGTCTAA23800.014.1126211
CGAATCG2353.080523E-513.9792445
GTACAGG43800.013.8366451
GCTCTAG34100.013.8112231
GTCCTAT27000.013.6569741
GTGCTAG34950.013.4753281
GTATAGG23400.013.4177421
GTGTTAG44700.013.2313561
GTCCTAC23250.013.1902541
GTACTAT30600.013.1240571
Produced by FastQC (version 0.11.5)