FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11504056
Sequences flagged as poor quality0
Sequence length79
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG22050.020.0337331
GTATTAG37100.017.6142541
GTCCTAG34450.016.7437611
GTACTAA32950.016.5088181
GGTCTAG26200.016.3030821
GCGAGAC45250.016.0518553
GTCCTAA30150.015.8624081
CGAGACT46100.015.4392544
CGCGTCA5450.014.73380915
GTCTTAG33400.014.6468251
GTGCTAG39350.014.1948831
GTTCTAG42000.013.8207931
GTGCAGG82850.013.527921
AGCGAGA50750.013.4491962
TACGCGA1900.001381015913.4473032
GCCCTAG29900.013.4309471
GCTCTAG34000.013.4219691
TCTAGAC34450.013.2437673
GCAAGAC83750.013.0745643
GTACAGG47850.013.04665851
Produced by FastQC (version 0.11.5)