FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11504056
Sequences flagged as poor quality0
Sequence length79
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG20150.019.008621
GTATTAG37450.017.9226551
GTGCTAG37000.016.8589571
GTCCTAG36350.016.3575971
GCGAGAC44400.016.267843
CTAGACT33100.015.8706724
CGAGACT46400.015.488494
GTCTTAG35450.015.4351681
GTCCTAA31050.014.9203321
GGTCTAG24750.014.4439921
GTGCAGA71850.014.1141371
GGTTTAG32400.013.8482991
CCTAGAC30400.013.7997733
GTATTAA58650.013.74550151
GGTCTAA27600.013.4811651
GTGCAGG84050.013.2806711
GTGTTAG43750.013.2573091
CTAGACC28100.013.2420344
GTACTAA32800.013.2345571
GTACTAT32300.013.2135551
Produced by FastQC (version 0.11.5)