FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10591834
Sequences flagged as poor quality0
Sequence length79
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG32100.017.8564151
GTGCTAG35950.017.0612181
GTCCTAG30200.016.924691
GTCTTAG29850.016.6339051
CGATCGT2251.1408574E-616.22153938
GGTCTAG23150.015.9283231
GTACTAG18800.015.9241291
GTTCTAG36650.014.9422821
GTATTAA52150.014.9116241
CTAGACC24100.014.6908784
GCGAGAC40150.014.636373
GTGTTAG41100.014.5680591
GTCCTAA30350.014.435181
CCTAGAC29400.014.2772163
GTTCTAA43300.014.1651461
CGAGACT41000.014.1548844
CGAATCG2605.1222323E-614.03932845
GTCGTAC4755.456968E-1213.8349811
GGTCTAA25150.013.6455011
GTGCAGG82550.013.4448561
Produced by FastQC (version 0.11.5)