FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10385607
Sequences flagged as poor quality0
Sequence length79
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18750.018.6973421
GTCCTAG32000.018.2591231
GCGAGAC39700.017.1013973
GTATTAG35100.017.0626561
GTTCTAG40900.016.1608871
CGAGACT41800.015.9801094
GTGCTAG31850.015.2493781
GTACTAA30900.015.12731
GTCCTAA30050.014.9475681
GTCTTAG30700.014.8689931
GAGCGAG45800.014.750821
GTATTAA49500.014.6810741
GCTCTAG31500.014.2595051
GCCTTAG29800.014.2151571
GTCGTAC3654.8785296E-914.0069991
GCCCTAG28600.013.9177921
CCTAGAC27100.013.7385233
AGCGAGA46850.013.6344482
GTGCAGG79850.013.5371331
GCAAGAC73750.013.5116923
Produced by FastQC (version 0.11.5)