FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13903272
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG28100.017.0688861
GTACTAG16100.016.961321
GTCCTAG32850.015.77310851
TACGCGA3602.7284841E-1115.5599582
GTTCTAG40850.015.169531
GTATATA53250.014.3326931
GTGCTAG33700.013.9208521
GTATAGG22950.013.8819231
TCTAGAC31250.013.7799483
GTACTAA28300.013.608091
GTATTAA45550.013.5274271
GTCTTAG29300.013.5021131
CTATACT34400.013.3325484
GTATAAG27850.013.0737171
GTATATG41850.012.966631
GTATAGA29350.012.882691
GTACAGG62350.012.8584881
GTCTAGA31050.012.8538791
GCTCTAG39250.012.6660041
TCTATAC31600.012.63023
Produced by FastQC (version 0.11.5)