FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13748217
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16300.018.2527541
GTCCTAG32850.016.302461
GTATTAG28050.016.0973341
GTCTTAG29600.015.7274051
GTTCTAG38750.015.175211
GTATAGG24550.014.6853011
GTATTAA45450.014.0933511
TAGTGCG7950.014.08781557
GTATATA50250.014.0705931
GTACTAA27000.013.8712991
GTTCTAA40700.013.7601351
CGATCGT5250.013.33340338
GTGCTAG31300.012.9721141
CTAGACT31700.012.6983354
GTACAGG62900.012.6876541
GTCTAGG31500.012.5563961
CGCGAGA9300.012.419147517
GTACATA38500.012.3644641
GTGCAGG116750.012.3520041
GTCCTAA28800.012.2751271
Produced by FastQC (version 0.11.5)