FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13748217
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18400.019.0573481
GTCCTAG32050.017.1771831
GTACTAA26550.016.773341
GTATTAA46300.016.1316551
TAGTGCG6950.015.610702557
GTCTTAG32450.015.5606641
GTTCTAG39950.015.4481411
GTATTAG25950.015.1342531
GCTCTAG38450.015.0476251
GTATATA49450.015.0331461
GTCCTAA31350.014.9881331
GGTCTAG24600.014.8244491
GTGCTAG34600.014.2897071
GGTCTAA26250.014.1597931
GCGATCG6250.013.99927637
TCTAGAC30850.013.9595323
GTTCTAA42500.013.8611961
TAGTCGG9600.013.853459
CGCGAGA9950.013.71788317
CCTATAC22950.013.5777643
Produced by FastQC (version 0.11.5)