FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13448368
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16250.019.6009671
GTATTAG28550.017.7767071
GTATTAA44550.015.7134581
GTTCTAG37600.015.266711
GCGATCG6250.015.12040837
CGATCGT5850.014.95766838
GTCCTAG33200.014.1272021
GCCCTAG29500.013.644741
GTATATA50950.013.4648451
GTATAAG29400.013.4530451
GTCTTAG30550.013.2903431
GTGCTAG28500.013.0181851
CTATACT33900.013.0096364
ATTAGAC28000.013.00068953
GTACTAT24550.012.831591
GCTCTAG38000.012.8032631
GTATATG40600.012.24198151
TATACTG40800.012.1821545
ATCTCCG99200.012.17216470
GTGTAGG43300.012.1253091
Produced by FastQC (version 0.11.5)