FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10698156
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13300.017.5498451
GTCCTAG27200.017.1627181
GTATTAG21200.015.7055031
TACGCGA1554.705027E-415.50246052
GTGCTAG28100.015.26932051
GTTCTAG32150.014.9473111
GTCCTAA23750.014.1637571
GTTCTAA29950.013.7530941
GTATAGG18000.013.7301731
GTACTAA21900.013.3226561
GTATTAA31300.013.2695751
GTCTTAG25150.013.1023521
GTATATA38400.012.9614271
TATACTG29950.012.9516785
TCTAGAC22650.012.88198953
CTATACT25200.012.3958744
GTACAAG34450.012.3551621
GTACATG42000.012.340811
GGTCTAG20000.012.1855281
GTGTAGG33750.012.1028931
Produced by FastQC (version 0.11.5)