FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10698156
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG27350.016.8711431
GTACTAG14350.016.0775531
GTATTAA32000.014.6347711
GTCCTAA23150.014.5771341
GTATTAG22400.014.2965731
GTCCTAG25400.014.2348411
TCTAGAC25000.014.1631483
GTACTAA19950.014.1536351
GTATATA37650.013.9934081
GCTCTAG32750.013.7739031
GCGCTAG3253.4711775E-813.7739021
GTATAAA42750.012.7267651
CTAGACC23000.012.5476394
GTATTAT31900.012.4137851
GTTCTAG31950.012.3943581
TATACTG31400.012.3579395
GTACATA26750.012.3578941
GTATAGG16850.012.2616351
GTTCTAA32100.012.2291671
GTCCTAT21150.012.2109071
Produced by FastQC (version 0.11.5)