FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10860360
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG23350.019.408351
GTATTAA30450.016.4614121
GTCTTAG26500.016.1944311
GTTCTAG30300.015.4098291
TATACGC3453.2923708E-1014.9279345
GTCCTAA22700.014.8218291
GTACTAG13750.014.731631
GTGCTAG27700.014.6252671
GTCCTAG28450.014.1190391
GGTCTAA20700.013.7660481
CTTATAC23300.013.7039143
GTTCTAA33350.013.6916991
GTACTAA19400.013.6266961
GCTCTAG30800.013.1532431
GTATATA40700.013.0749131
GTGCATA26000.012.6765021
TTATACT35000.012.4583124
GTACAAG37000.012.4338221
GTATATG33750.012.4104521
GTACCGT7250.012.3133136
Produced by FastQC (version 0.11.5)