FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10753142
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22200.015.1554471
GTCCTAG27350.014.6866991
GTACTAA18950.014.4936051
GTGTTAG28550.013.9491431
TCTATAC22950.013.91323953
GTGCTAG24800.013.7050061
TATACTG31650.013.6690055
GTTCTAA30600.013.4634231
GTACTAC15950.013.3452481
GTATATA40000.013.3035451
TCTGTAC44500.013.2707793
GTTCTAG32400.013.2452661
GTCCTAT20850.013.1728451
GTATTAA31050.013.0471621
CTATACT25950.012.8341294
GGTCTAA21600.012.7154551
GTACAGG46800.012.6176431
TCTAGAC25050.012.6097963
GTATAGG17350.012.4662751
GTCTTAA29650.012.3895261
Produced by FastQC (version 0.11.5)