FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10753142
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG30050.016.0081621
GTATATA38450.015.817391
GGTCTAG18600.015.7023231
GTGCTAG27650.015.6578921
GTACTAG11500.015.5368671
GTACTAA20850.015.491011
GTCCTAG26800.015.2570331
GTCCTAA23400.015.124441
GTATTAA29900.014.2497871
GTACATA30850.014.1451161
GCCCTAG25650.014.0656521
GTATTAG19900.013.9858831
GTCTTAG26350.013.6919911
GTACTAT20150.013.3007921
CCTCTAG43250.013.2674751
GTTCTAA32250.013.21143151
GTGCTAA27850.013.2012931
CTCCTAG43050.012.93003751
GTATTAT31500.012.6533461
GTGTTAG29100.012.5161511
Produced by FastQC (version 0.11.5)